Yes, but of course there are two lengths of cartos, a shorter one for smaller 3ml tanks and a longer one for larger 5ml + tanks. I actually use longer ones in some of my shorter tanks, which is fine, too. The other way around won't work, though.
Also, you'll need a carto punch tool to punch your holes. I recently picked up something called a saddle valve punch at a local hardware store for around $8 with tax. Works great! I'm leaning more towards two holes in my cartos these days. Or, you could just order your cartos "pre-punched."