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"Methods
ECS Exposure. A total of 85 male FVB/N mice (6 to 8 wk old; The Jackson Laboratory) were randomly placed into 3 groups. One group (n = 45) was exposed to ECS generated from e-juice (nicotine [36 mg/mL] dissolved in vehicle [Veh; isopolypropylene glycol and vegetable glycerin at a 1:1 ratio]). We maintained the particulate matter concentration in the chamber at 130 mg/m3 and the aerosol nicotine concentration at 0.196 mg/m3 (SI Appendix, Table S1). The second group (n = 20) was exposed to Veh. Aerosols for both groups were generated using an automated 3-port E-cig aerosol generator (e∼Aerosols) set at a constant voltage (1.9 A, 4.0 V) (SI Appendix, Table S1), the same as is done in commercial E-cigs (12, 13). Mice were subjected to whole-body exposure. The exposure conditions were the same as previously described (12). Mice were exposed for 4 h per day and 5 d per week for 54 wk. The third group (n = 20) remained housed in the animal room, exposed to the ambient filtered air (FA). During the 54-wk period, 3 ECS mice were found dead and 2 ECS mice had to be killed because of inactiveness. No lung tumor was observed in these 5 mice, and 1 was found to have a large intestinal polyp. One Veh-exposed mouse was found dead, and 1 was killed due to a paralyzed leg. Two FA-exposed mice were also found dead. No lung tumor was observed in these 2 Veh and 2 FA mice. At the end of the 54-wk exposure, 40 ECS-exposed, 18 Veh-exposed, and 18 FA-exposed mice survived. The average body weights among these 3 groups were similar (FA group, 34.4 ± 5.84 g; Veh group, 34.0 ± 2.78 g; and ECS group, 35.1 ± 2.99 g; ECS vs. FA, P = 0.67; ECS vs. Veh, P = 0.1998), and all mice appeared healthy. These mice were killed to examine tumor formation in different organs."
So what's the Take-Away?
Don't Lock yourself in a Phone Both for 4 Hours where 36mg/ml Vapor is being pumped in?
ECS Exposure. A total of 85 male FVB/N mice (6 to 8 wk old; The Jackson Laboratory) were randomly placed into 3 groups. One group (n = 45) was exposed to ECS generated from e-juice (nicotine [36 mg/mL] dissolved in vehicle [Veh; isopolypropylene glycol and vegetable glycerin at a 1:1 ratio]). We maintained the particulate matter concentration in the chamber at 130 mg/m3 and the aerosol nicotine concentration at 0.196 mg/m3 (SI Appendix, Table S1). The second group (n = 20) was exposed to Veh. Aerosols for both groups were generated using an automated 3-port E-cig aerosol generator (e∼Aerosols) set at a constant voltage (1.9 A, 4.0 V) (SI Appendix, Table S1), the same as is done in commercial E-cigs (12, 13). Mice were subjected to whole-body exposure. The exposure conditions were the same as previously described (12). Mice were exposed for 4 h per day and 5 d per week for 54 wk. The third group (n = 20) remained housed in the animal room, exposed to the ambient filtered air (FA). During the 54-wk period, 3 ECS mice were found dead and 2 ECS mice had to be killed because of inactiveness. No lung tumor was observed in these 5 mice, and 1 was found to have a large intestinal polyp. One Veh-exposed mouse was found dead, and 1 was killed due to a paralyzed leg. Two FA-exposed mice were also found dead. No lung tumor was observed in these 2 Veh and 2 FA mice. At the end of the 54-wk exposure, 40 ECS-exposed, 18 Veh-exposed, and 18 FA-exposed mice survived. The average body weights among these 3 groups were similar (FA group, 34.4 ± 5.84 g; Veh group, 34.0 ± 2.78 g; and ECS group, 35.1 ± 2.99 g; ECS vs. FA, P = 0.67; ECS vs. Veh, P = 0.1998), and all mice appeared healthy. These mice were killed to examine tumor formation in different organs."
So what's the Take-Away?
Don't Lock yourself in a Phone Both for 4 Hours where 36mg/ml Vapor is being pumped in?
"Research was supported by NIH Grants,RO1190678, 1PO1CA165980, P30CA16087, and ES00260."
They got the grants because NIH knew they were reliable anti-tobacco ideologues, and would concoct the desired results. This is the real corruption of research that the hypocrites who triumphantly point at "tobacco industry money" completely ignore. Their media accomplices treat them as beyond even a thought of questioning. Never mind that decades of junk science on cigarette smoking didn't establish nicotine as a carcinogen.
They got the grants because NIH knew they were reliable anti-tobacco ideologues, and would concoct the desired results. This is the real corruption of research that the hypocrites who triumphantly point at "tobacco industry money" completely ignore. Their media accomplices treat them as beyond even a thought of questioning. Never mind that decades of junk science on cigarette smoking didn't establish nicotine as a carcinogen.
No lung tumors found in any of these mice and given that they even had 2 mice die breathing filtered ambient air, that would seem to point to us being doomed no matter what we do. 
Hey, nobody gets out of this thing alive, lol.
Hey, nobody gets out of this thing alive, lol.
I'm missing the type of coil they were using and also if they changed the coils and cotton.
No, it points to these mice being doomed. One thing rarely mentioned:No lung tumors found in any of these mice and given that they even had 2 mice die breathing filtered ambient air, that would seem to point to us being doomed no matter what we do.
The mice in used in these sorts of studies have been selectively bred for extreme susceptibility to cancers.
Then there's the context missing from this study: A fourth control group that was exposed to cigarette smoke at the same level as the mice exposed to e-cig vapor should have been included. Wanna bet those mice would all have been dead before the study ended?
Oh, and lastly, the skeptic in me would like to examine the equipment used to produce vapor for four hours a day.
Obviously they needed a way to attract the younger mice, as no adult mouse could possibly be interested in vaping cheese flavored.
Exactly! I was trying to point out, in a sarcastic yet humorous fashion, that attempting to draw meaningful conclusions from that study was essentially futile. I may not have succeeded but at least it got you to bring forth some great points.
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