I have taken one new sideline 'experiment' with the initial tobacco preparation that I hope will dramatically increase the extraction efficiency of the alkaloids from the tobacco matrix.
zyxel-nsa210.lilu2.ch/MyWeb/public/.../Q10%20in%20solanesol.pdf
discusses the Microwave-assisted extraction of solanesol from tobacco leaves.... the electron photographs show cell wall degradation from moderate amounts of microwave radiation.
by the mechanism of action, we should be able to achieve the same results without using a microwave.
Basic premise, would be to
1. add sufficient basic solution to cause sufficient cellular turgidity.
2. pre-heat the mineral oil to slightly greater than 100C.
3. slowly add the tobacco to the oil, and the oil would flash boil the water in the tobacco
This would cause cell wall rupturing, and expel the cell products with enough energy to escape the tobacco matrix, and dissolve into the oil.
4. The excess water would be boiled off, maximizing the partition co-efficient.
* limitation would be that the excess carbonate would have to be carefully filtered to prevent it from neutralizing the acidic water step.
zyxel-nsa210.lilu2.ch/MyWeb/public/.../Q10%20in%20solanesol.pdf
discusses the Microwave-assisted extraction of solanesol from tobacco leaves.... the electron photographs show cell wall degradation from moderate amounts of microwave radiation.
by the mechanism of action, we should be able to achieve the same results without using a microwave.
Basic premise, would be to
1. add sufficient basic solution to cause sufficient cellular turgidity.
2. pre-heat the mineral oil to slightly greater than 100C.
3. slowly add the tobacco to the oil, and the oil would flash boil the water in the tobacco
This would cause cell wall rupturing, and expel the cell products with enough energy to escape the tobacco matrix, and dissolve into the oil.
4. The excess water would be boiled off, maximizing the partition co-efficient.
* limitation would be that the excess carbonate would have to be carefully filtered to prevent it from neutralizing the acidic water step.
