Cell harm seen in lab tests of e-cigarettes...

[guyvelella]

http://medicalxpress.com/news/2015-12-cell-lab-e-cigarettes.html
E-Cigarettes Could Actually Cause Cancer, Study Finds
Shocking cancer claims could put you off using e-cigarettes
Cell harm seen in lab tests of e-cigarettes

Whats your thoughts on this new study?

 

[WillyZee]

quote: Study did not mimic actual doses

 

[Robert Cromwell]

Replace the ecig juice with MT Dew and the cell damage might be worse.
Heck Milk might damage cells in that application.

 

[sofarsogood]

If real vaping hazards exist nobody is going to be more interested than people who vape. I keep an eye out for reports of doctors treating illnesses caused by using ecigs. Anybody seen anything? The only voice in public health research I trust is Dr. F. The rest of them might as well shut up because I'm not listening any more. Click.

 

[Rossum]

The overarching question is whether the battery-operated products are really any safer than the conventional tobacco cigarettes they are designed to replace.

Wang-Rodriquez doesn't think they are.

"Based on the evidence to date," she says, "I believe they are no better than smoking regular cigarettes."

Dear Ms. Wang-Rodriquez: Please repeat your study and compare actual cigarette smoke to vapor on your cells and let us know if you still think that.

Or maybe just climb down from your ivory tower and talk to some people who were heavy smokers for decades and switched to vaping.

 

[inspects]

"In this particular study, it was similar to someone smoking continuously for hours on end, so it's a higher amount than would normally be delivered," she says.

Unbelievable what some Idiots will do for misinformation.

 

[puffon]

"Based on the evidence to date," she says, "I believe they are no better than smoking regular cigarettes."

 

[Painter_]

This seems excessive on first glance and was this vapor form or liquid form?

MATERIALS AND METHODS:
HaCaT, UMSCC10B, and HN30 were treated with nicotine-containing and nicotine-free vapor extract from two popular e-cigarette brands for periods ranging from 48h to 8weeks. Cytotoxicity was assessed using Annexin V flow cytometric analysis, trypan blue exclusion, and clonogenic assays. Genotoxicity in the form of DNA strand breaks was quantified using the neutral comet assay and γ-H2AX immunostaining.

Link to the actual study http://www.oraloncology.com/article/S1368-8375(15)00362-0/fulltext I am still reading the study but thought others would like to see it as well.

 

[cags]

I'm pretty sure my cells are already messed up from the poison they put in and on our foods and in our medicines, in our air, etc, etc.........

 

[DrMA]

http://medicalxpress.com/news/2015-12-cell-lab-e-cigarettes.html
Cell harm seen in lab tests of e-cigarettes

Whats your thoughts on this new study?

Here's how you interpret cell studies properly:
Cell studies on e-cigarettes: don’t waste your time reading (at least most of) them

Sent from my HTC One M9 using Tapatalk

 

[curiousJan]

Uhm ...

"Treatment media was replaced every three days with 1% e-cigarette extract. Because of the high toxicity of cigarette smoke extract, cigarette-treated samples of each cell line could only be treated for 24h."

So the cigarette treated samples were dead after 24 hours, but ecigs are no safer? Good Grief!

 

[defdock]

Again with the false information...

When the hell did I start putting formaldehyde in my ejuice?

"Scientists already know of some troubling chemicals in the products. One is formaldehyde, a known carcinogen"

 

[CarolT]

Here's how you interpret cell studies properly:
Cell studies on e-cigarettes: don’t waste your time reading (at least most of) them

Sent from my HTC One M9 using Tapatalk

Great article. This is the same kind of BS they've been doing in anti-smoking propaganda for decades, too. The 0.03mg/L nicotine concentration in blood of smokers versus the >16mg/L in the experiment shows how bogus it is.

 

[EBates]

So, the VA can afford to fund another BS worthless rehashed 'cell damage' study, that was discredited over 6 months ago, but can't treat the Vets that Defended Our Country in the BS Wars that our Precious Govmint throws them into?

Makes you Proud to be an American, don't it!

 

[CarolT]

Its biggest fraud is in insinuating that their assays prove that e-cigs can cause cancer. "One of the most pertinent questions regarding the relative safety of e-cigs is whether or not e-cigs have the potential to cause DNA damage in human cells," they slime. But those assays have not been validated to be able to predict this! And in fact, a similar Comet assay was shown by a prospective study NOT to do so!

In Epstein-Barr virus-transformed lymphoblastoid cell lines established from prospectively collected peripheral blood samples of 117 lung cancer patients with 117 matched controls, the alkaline Comet assay and the host cell reactivation (HCR) assay with the mutagen benzo[a]pyrene diol epoxide were unrelated to lung cancer risk. In the bleomycin mutagen sensitivity assay, "statistically significantly increased lung cancer odds ratios (OR(adjusted)) were observed for bleomycin mutagen sensitivity as quartiles of chromatid breaks/cell [relative to the lowest quartile, OR = 1.2, 95% confidence interval (CI): 0.5-2.5; OR = 1.4, 95% CI: 0.7-3.1; OR = 2.1, 95% CI: 1.0-4.4, respectively, P(trend) = 0.04]. The magnitude of the association between the bleomycin assay and lung cancer risk was modest compared with those reported in previous lung cancer studies but was strengthened when we included only incident cases diagnosed more than a year after blood collection (P(trend) = 0.02), supporting the notion the assay may be a measure of cancer susceptibility." [However, infections by both Epstein-Barr virus and cytomegalovirus make cells more susceptible to damage by bleomycin.]
Prospective analysis of DNA damage and repair markers of lung cancer risk from the Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Trial

 

[VNeil]

Replace the ecig juice with MT Dew and the cell damage might be worse.
Heck Milk might damage cells in that application.

And that Sir, is precisely why I replaced that toxic Mountain Dew with Pepsi.

(there is an on point analogy here )

 

[AndriaD]

And that Sir, is precisely why I replaced that toxic Mountain Dew with Pepsi.

(there is an on point analogy here )

+1 for Pepsi.

Andria

 

[VNeil]

+1 for Pepsi.

Andria

Oh, reminds me I did a back of the envelope calculation of the equivalent human dosage of the infamous Diacetyl rat tests. I calculated the equivalent dosage to be 3 MILLION micrograms. That is 150x the dosage of the very worst case extreme vaping I could reasonably come up with... 1000 µg/ml X 20 ml per day....

Yet some people put more stock in that experiment than the real world experience of millions of vapers and one billion smokers.

Perhaps I made a mistake and someone will come up with a different number?

 

[AndriaD]

Oh, reminds me I did a back of the envelope calculation of the equivalent human dosage of the infamous Diacetyl rat tests. I calculated the equivalent dosage to be 3 MILLION micrograms. That is 150x the dosage of the very worst case extreme vaping I could reasonably come up with... 1000 µg/ml X 20 ml per day....

Yet some people put more stock in that experiment than the real world experience of millions of vapers and one billion smokers.

Perhaps I made a mistake and someone will come up with a different number?

I think it's probably right. My own avoidance of them, as previously noted, has more to do with asthma, hypochondria, and anxiety, than any real fear that it's a real threat. My own brain is a far bigger threat to my peace of mind than anything in ejuice.

Oh, and just let me say: +2 for Dr Pepper.

Andria

 

[ruet]

Here's the important part folks: "The damage occurred even with nicotine-free versions of the products." It's on now. They don't want us vaping anything. ...ever! All the counter arguments we make about reducing nic to 0 or vaping nic-free juice to begin with are going to be systematically dismantled with garbage like this. Look for this "study" to be quoted ad-nauseum.