Development of an in vitro cytotoxicity model for aerosol exposure using 3D reconstructed human airway tissue; application for assessment of e-cigarette aerosol
This study demonstrates the applicability of physiologically relevant EpiAirway™ tissue used in combination with a VITROCELL® VC 01 exposure system for the assessment and comparison of cigarette smoke and e-cigarette aerosol. Despite being tested with a more intense puffing regime, e-cigarette aerosol showed no acute cytotoxicity in this study when compared with traditional 3R4F reference cigarette smoke. Under the study conditions cigarette smoke demonstrated a dose-dependent response that resulted in near-complete cell death after a 6 h exposure period. In contrast, e-cigarette aerosol showed no decrease in tissue viability following a 6 h exposure, despite appropriate positive control responses. Furthermore, cytotoxicity appears to be unaffected by different e-cigarette formulations as tested in this study. Further studies will need to be conducted to compare between different commercially available products, formats, and formulations, but our data suggest that e-cigarette aerosols have significantly less impact than cigarette smoke over the duration of a 6 h exposure in vitro using organotypic tissue constructs.
This study demonstrates the applicability of physiologically relevant EpiAirway™ tissue used in combination with a VITROCELL® VC 01 exposure system for the assessment and comparison of cigarette smoke and e-cigarette aerosol. Despite being tested with a more intense puffing regime, e-cigarette aerosol showed no acute cytotoxicity in this study when compared with traditional 3R4F reference cigarette smoke. Under the study conditions cigarette smoke demonstrated a dose-dependent response that resulted in near-complete cell death after a 6 h exposure period. In contrast, e-cigarette aerosol showed no decrease in tissue viability following a 6 h exposure, despite appropriate positive control responses. Furthermore, cytotoxicity appears to be unaffected by different e-cigarette formulations as tested in this study. Further studies will need to be conducted to compare between different commercially available products, formats, and formulations, but our data suggest that e-cigarette aerosols have significantly less impact than cigarette smoke over the duration of a 6 h exposure in vitro using organotypic tissue constructs.