Some info on testing here https://www.nikitest.com/e-liquid-nicotine-test-lab/e-liquid_test_kit_works/
Basically you use acid to neutralize the nicotine, the amount of acid use is proportional to the amount of nic in the sample being tested.
Say I have 0.1N acid ( which can be made by adding 12ml of 5% vinegar in 100ml of distilled water). I put 1 ml of " supposed" 18mg /ml juice in a test vial. I then added 2 ml of distilled water to the vial and 4-5 drops of pH test liquid (6 - 7.2, can be found at most pet store that carry fish). The solution will be blue. Slowly add the acid; it will turn green then yellow. When it turns yellow, stop. Multiply the amount of acid used by 16.22. That is the amount of nic in the vial. The more nicotine you test the more accurate the result.
This test method was developed by DVAP, all credits goes to him. Search this forum for more information.
Note that 16.22 is used above is because my acid is 0.1N and not 0.12N.
Great, thanks a lot for posting this!