I just got an order of 36 mg/ml from Eastmall and it came in 10 ml bottles. I happen to have a number of bottles now of 36 mg, so I tossed one in the freezer (set at 0F/-18C). I'll get it tested next week. I will post about the efficacy of this.
Test parameters:
1. Does niquid freeze? (Funny enough, this hasn't been answered yet.)
2. Does it melt without noticeable damage to a) appearance (color, clarity, viscosity)? b) nicotine content? (testing by HPLC, comparing unfrozen sample to frozen and defrosted sample.)
3. Can the bottles withstand freezing?
Anything else I should look for? (In terms of long-term storage, if freezing damages the liquid, that will be obvious after a few days in the freezer, and storage at -18C is usually enough to prevent degradation.) The HPLC report will give me a content analysis of the two samples (i.e. I can see if those two samples contain anything besides nicotine, distilled water, PG and glycerol (glycerine)) though two data points do not prove anything.
(Yes, I know having access to a high performance liquid chromatograph lets me play with a piece of equipment most people don't have, and yes, I'd LOVE to test a sample of every niquid I can get my hands on, but I'm getting these two samples tested because somebody in the organic chem lab owes me a huge favor (of the get up in the middle of the night and drive 40 miles in pouring rain type) and won't likely happen again. I'm trying really hard to interest one of our shared brand new grad students in the testing project, and if so, I'll let this community know it's happening, but if I do get someone interested, I can't publish results until after zie publishes in peer review and that won't be soon. I'm also working on getting another grad student interested in testing blood levels of nicotine, cotinine and free dopamine in smokers, non-smokers, NRT users and vapers, but funding that study is going to be painful -- our normal funding sources won't pay for it, we'd need at minimum 50 of each test subject and a minimum 2 blood draws each (because the spit tests don't show levels, just presence of cotinine), and getting our alternative funding sources (drug companies) interested is going to take work -- so don't expect much.)
Test parameters:
1. Does niquid freeze? (Funny enough, this hasn't been answered yet.)
2. Does it melt without noticeable damage to a) appearance (color, clarity, viscosity)? b) nicotine content? (testing by HPLC, comparing unfrozen sample to frozen and defrosted sample.)
3. Can the bottles withstand freezing?
Anything else I should look for? (In terms of long-term storage, if freezing damages the liquid, that will be obvious after a few days in the freezer, and storage at -18C is usually enough to prevent degradation.) The HPLC report will give me a content analysis of the two samples (i.e. I can see if those two samples contain anything besides nicotine, distilled water, PG and glycerol (glycerine)) though two data points do not prove anything.
(Yes, I know having access to a high performance liquid chromatograph lets me play with a piece of equipment most people don't have, and yes, I'd LOVE to test a sample of every niquid I can get my hands on, but I'm getting these two samples tested because somebody in the organic chem lab owes me a huge favor (of the get up in the middle of the night and drive 40 miles in pouring rain type) and won't likely happen again. I'm trying really hard to interest one of our shared brand new grad students in the testing project, and if so, I'll let this community know it's happening, but if I do get someone interested, I can't publish results until after zie publishes in peer review and that won't be soon. I'm also working on getting another grad student interested in testing blood levels of nicotine, cotinine and free dopamine in smokers, non-smokers, NRT users and vapers, but funding that study is going to be painful -- our normal funding sources won't pay for it, we'd need at minimum 50 of each test subject and a minimum 2 blood draws each (because the spit tests don't show levels, just presence of cotinine), and getting our alternative funding sources (drug companies) interested is going to take work -- so don't expect much.)
And yes, I know it has to be much colder to freeze solid, but I'd say only 10% or so of vapers have access to the type of cold storage that would actually freeze the stuff, while almost all of us have access to a household freezer. *THAT* I did take into consideration. 
