Beta Carboline MAOIs - towards a more effective e-liquid

[tescela]

when is vaping not an option? i do so when and where i please, if i think there might be a problem i ask if there is then i promise them they will not catch me doing it there then (and they won't i'm a good stealther) it's not hard to keep something small w/ u for extra carts, batts, juice etc.

plus there are those of us who have no interest in quitting but tried this and find it a better alternative. i wouldn't find it near as enjoyable as a nasal spray or something i couldn't mess around w/. i think that's y i liked RYOs when smoking cause it gave me something i could do w/ my hands. for me this has turned into a hobby. i just want more effective stuff cause i'm noticing that my alternative is going on anti-depressants and/or other medications that will kill me faster than smoking ever dreamed of.

Demon Cowboy, different strokes for different folks. Many would ask why bother with any of this stuff at all when we have E-Juice.
For them, nic alone is enough... and for others, 'vaping' is enough, so why bother looking at alternatives.

besides the bother of having to fill carts, recharge batteries etc... there are places that I cannot vape, including where I work. There, I can't use gum or lozenges in certain areas, so have to 'stealth chew'. 'stealth vaping" would be somewhat more difficult, and I don't wish to make vaping a source of anxiety for myself.

Thank you for responding to this so well, tceight.

DemonCowboy, this isn't about asking permission or forgiveness. This thread (see below) will clear up any potential misconception there...

http://www.e-cigarette-forum.com/forum/campaigning-discussions/116299-bans-endgame-way-forward.html


Instead, this is about enlarging our toolkit to address situations in which vaping simply isn't practical (e.g., while performing surgery, working in a "clean room," working on a factory line, etc.) or we would prefer not to vape.

Moreover, I reiterate my other thought, which was that it would seem that there would be a lot of overlap in research (i.e., learning about one could help you learn how to improve the other).

One last thing: would it be a good idea for one of the more chemistry-gifted members of this group to try using CigRx to make a vapable WTA-like eliquid? Regardless of the outcome, the process would likely provide some valuable insight, right?

 

[tceight]

One last thing: would it be a good idea for one of the more chemistry-gifted members of this group to try using CigRx to make a vapable WTA-like eliquid? Regardless of the outcome, the process would likely provide some valuable insight, right?

I think this would be invaluable info. I couldn't find a monograph for it online, and so asked Jamesam in post 430 if one came with his package. If we can find out the mg per pill of anatabine, then there is a good starting point for dilutiont. Assuming the inert carrier materials are water soluble and the concentration is high enough, simply dissolving a CigRx pill in the correct amount of nic-juice would give immediate insight to whether this line of experimentation is worth pursuing.
As for a long term solution to the 'end game scenario'?? I don't expect CigRx to be around long.

 

[kinabaloo]

just speaking for yourself, or do you wish this thread not to discuss alternate titration methods of beta carbolines?
it is titled, " Beta Carboline MAOIs - towards a more effective e-liquid"

I personally am not married to vaping, and would love the idea of a WTA sub-lingual tab, or nasal spray if it had the efficacy and accuracy of vaping.

I guess I was a little curt. But I was saying, via my own preferences, why there's not much interest in the pill among vapers - there's no pleasure in a popping a pill.

As the thread starter, of course I am interested in the composition and effects and expect even a single beta carboline, if the right one (or from several right ones) to be the key 'missing'. Hence I have positively supported both tobacco extraction and experiments with nic liquid plus beta carboline (PF extract for example). About 1-2% of the total alkaloid content (effectively 1-2% as much carboline as nic) should be about right, from the amounts absorbed from smoking.

Hope that's clear now

 

[kinabaloo]

Here is a list of MAO-A and MAO-B inhibitors, for reference.

• Selective MAO-A inhibitors
  • Befloxatone
  • Brofaromine (Consonar)
  • Cimoxatone
  • Clorgyline
  • Curcumin (found in turmeric)
  • Harmala alkaloids (found in tobacco, syrian rue, passion flower, and ayahausca)
    • Harmine
    • Harmaline
    • Tetrahydroharmine
  • Methylene Blue
  • Minaprine (Cantor)
  • Moclobemide (Aurorix, Manerix)
  • Pirlindole (Pirazidol)
  • Toloxatone (Humoryl)
  • Tyrima (CX157)
• Selective MAO-B inhibitors
  • Catechin (found in cat's claw)
  • Desmethoxyyangonin (found in kava kava)
  • Epicatechin (also found in cat's claw)
  • Fo-Ti (active constituent unknown)
  • Hydroxytyrosol (found in olive oil)
  • Lazabemide
  • Pargyline (Eutonyl)
  • Piperine (found in pepper)
  • Rasagiline (Azilect)
  • Selegiline (Deprenyl, Emsam)

Natural MAO-B inhibitors? - Mind and Muscle Forums

A further list, this time primarily botanicals, with some non-overlaps :

◦Selective MAO-A Inhibitors
■Resveratrol (found in skin of red grapes / wine) [13]

◦Nonselective MAO-A/MAO-B Inhibitors
■Curcumin (found in turmeric / curries)
■Harmala alkaloids (found in tobacco, syrian rue, passion flower, ayahausca, and Tribulus terrestris)
■Harmine, Harmaline, Tetrahydroharmine, Harmalol, Harman, Norharman, etc
■Rhodiola Rosea (active consituent(s) unknown)

◦Selective MAO-B inhibitors
■Catechin (found in the tea, cocoa, and cat's claw)
■Desmethoxyyangonin (found in kava)
■Epicatechin (also found in the tea plant, cocoa, and cat's claw)
■Fo-Ti (active constituent(s) unknown)
■Hydroxytyrosol (found in olive oil)
■Piperine (found in pepper)

◦Unknown Selectivity
■Ginkgo Biloba (active constituent(s) unknown)
■Liquorice (active constituent(s) unknown)
■Myristicin (found in nutmeg, parsley, and dill)
■Siberian Ginseng (active constituent(s) unknown)
■St. John's Wort (active constituent(s) unknown)
■Yerba Mate (active constituent(s) unknown)
■Yohimbe (active constituent(s) unknown)

http://wapedia.mobi/en/Monoamine_oxidase_inhibitor

"In addition to reversibility, MAOIs differ by their selectivity of the MAO receptor. Some MAOIs inhibit both MAO-A and MAO-B equally, other MAOIs have been developed to target one over the other.

MAO-A inhibition reduces the breakdown of primarily serotonin, epinephrine, and norepinephrine and thus has a higher risk of serotonin syndrome and/or a hypertensive crisis. Tyramine is broken down by MAO-A, therefore inhibiting its action may result in excessive build-up of it, so diet must be monitored for tyramine intake.

MAO-B inhibition reduces the breakdown mainly of dopamine and phenethylamine so there are no dietary restrictions associated with this. Two such drugs, selegiline and rasagiline have been approved by the FDA without dietary restrictions, except in high dosage treatment where they lose their selectivity.[2][4]

http://en.wikipedia.org/wiki/Monoamine_oxidase_inhibitor

-------

"Monoamine oxidase (MAO) is a mitochondrial outer-membrane flavoenzyme involved in brain and peripheral oxidative catabolism of neurotransmitters and xenobiotic amines, including neurotoxic amines, and a well-known target for antidepressant and neuroprotective drugs. Recently, positron emission tomography imaging has shown that smokers have a much lower activity of peripheral and brain MAO-A (30%) and -B (40%) isozymes compared to non-smokers. This MAO inhibition results from a pharmacological effect of smoke, but little is known about its mechanism. Working with mainstream smoke collected from commercial cigarettes we confirmed that cigarette smoke is a potent inhibitor of human MAO-A and -B isozymes. MAO inhibition was partly reversible, competitive for MAO-A, and a mixed-type inhibition for MAO-B. Two beta-carboline alkaloids, norharman (beta-carboline) and harman (1-methyl-beta-carboline), were identified by GC-MS, quantified, and isolated from the mainstream smoke by solid phase extraction and HPLC. Kinetics analysis revealed that beta-carbolines from cigarette smoke were competitive, reversible, and potent inhibitors of MAO enzymes. Norharman was an inhibitor of MAO-A (K(i)=1.2+/-0.18muM) and MAO-B (K(i)=1.12+/-0.19muM), and harman of MAO-A (K(i)=55.54+/-5.3nM). beta-Carboline alkaloids are psychopharmacologically active compounds that may occur endogenously in human tissues, including the brain. These results suggest that beta-carboline alkaloids from cigarette smoke acting as potent reversible inhibitors of MAO enzymes may contribute to the MAO-reduced activity produced by tobacco smoke in smokers. The presence of MAO inhibitors in smoke like beta-carbolines and others may help us to understand some of the purported neuropharmacological effects associated with smoking."

Herraiz T, Chaparro C.
Spanish Council for Scientific Research, CSIC,
Biochem Biophys Res Commun. 2005 Jan 14;326(2):378-86

 

[tceight]

I was thinking some more about the sequential extraction numbers I was calculating, and they assume only partitioning the pg/vg.

If you divided up the mineral oil into several batches,

O1, O2, O3, O4, O5 etc,
and the PG into several..
A1, A2, A3, A4, A5 etc.

then mixed a1 with O1, then O2, etc..
then mixed a2 with O1, then O2, etc..
etc... etc..

you get far better extraction. This would be tedious as hell though to do manually, but I just made up an 'experiment' that seems to work well for doing this automatically. It may be possible to get a decent yield with NO acidification of the PG/Glycerine if the freebase alkaloids are soluble in the PG/VG.

I took 4 feet of 3/8inch ID clear vinyl tubing, wrapped it around a 2 inch pipe in a spiral and clamped the bottom closed.

about 30 ml of mineral oil, to which I had added food colouring. It didn't dissolve, but shaking it up well turned the mineral oil blue by holding tiny droplets in suspension.

put this in the tube, and then dripped 0.5 ml of a 75%VG 25% water mixture into the top.
It took a lot of messing around, to get the slope right. too steep and the VG travels quickly, not enough and it 'sticks' to the walls of the tube and doesn't move. (vapour locking is also a concern if you are not careful when adding) 45-50 degrees seemed to work.

once the VG droplet/bubble sank about 1 inch into the mineral oil, I added another .5ml, then another and another so that I had a train of vg bubbles sweeping through the mineral oil.
You could see how the first bubbles to get to bottom were a deep blue, and the successive bubbles were fainter and fainter.

after about 3 or 4 bubbles, (2ml), they rest were much much clearer, because the food colouring has a much higher (1000:1 ???? guessing here) affinity for the VG, but the principle holds. I believe in the lab this is called "counter-current separation"?

sounds complicated, but was easy to set up and this also makes the separation and final recovery of the VG as simple as slightly loosening the clamp, dripping slowly into your vial.

2$ of tube replaces the flasks, syringes, and or separatory funnels, takes far less 'hands on' time, and should be more efficient as well with what we have to work with.

If I can set this up right, I can have the entire apparatus run 'hands off', mineral oil seeping through the prepared tobacco, then dripping through an acid salt bed to dry/neutralize, then dripping directly into the countercurrent tube over night.

Once that is done, then setup a means to automatically drip the VG at the correct rate into the countercurrent tube, and come back in an hour to claim your prize.

Hope that's clear. I should take and post some pictures.

 

[tceight]

Here is a list of MAO-A and MAO-B inhibitors, for reference.

Nice compilation.
numerous of these also have other well known health benefits.
ie. curcumin is anti-inflammatory, and hydroxytyrosol is a powerful antioxidant.
Long term focus, a 'better than WTA' vape is certainly a probability.

 

[kinabaloo]

Nice compilation.
numerous of these also have other well known health benefits.
ie. curcumin is anti-inflammatory, and hydroxytyrosol is a powerful antioxidant.
Long term focus, a 'better than WTA' vape is certainly a probability.

Herbs and spices are generally a good nutrient source and packed with various types of anti-oxidants. I am a prodigous user of olive oil and peppercorns; and red wine, cocoa and tea. Sadly though the actives may not be that vapable, but I'd have to agree that a better WTA is in theory certainly possible.

Btw, curcumin has shown good anti-tumor activity too.

The one which i found had a noticeable effect was Ginko Biloba, one of the oldest living species on the planet; it markedly improves memory recall by improving blood flow; perhaps by making blood less sticky or perhaps by reducing plaque build-up.

----

If that ingenious appartus really extracts as efficiently this would be a great method. But seems it would not be able to transfer so well without the creation of an emulsion (tens of thousands of bubbles) that is then left to settle out for much longer. But hey, if it works - great !

 

[tceight]

duplicate post

 

[tceight]

(tens of thousands of bubbles)

that was my first thought, and initial attempt with the food colouring experiment was to use tiny droplets to increase surface area. It worked magnitudes better by increasing the bubble size to 'fill' the tube cross section. I think because this forces the mineral oil to pass by the bubble in a molecularly thin layer. If the alkaloids are even slightly hydrophilic, they will make the jump. if they are not, then diffusion will bump them across. If they miss the train, or the car is too full already, there is another less full one right behind it, with less passengers left to pick up.

 

[kinabaloo]

that was my first thought, and initial attempt with the food colouring experiment was to use tiny droplets to increase surface area. It worked magnitudes better by increasing the bubble size to 'fill' the tube cross section. I think because this forces the mineral oil to pass by the bubble in a molecularly thin layer. If the alkaloids are even slightly hydrophilic, they will make the jump. if they are not, then diffusion will bump them across. If they miss the train, or the car is too full already, there is another less full one right behind it, with less passengers left to pick up.

Later I was wondering if that was the case - as you describe. It' brilliant ! Excellent idea

 

[tceight]

laziness is the mother of invention, so I am a real "MacGyver" type.
For it to be practical, home based WTA extraction has to be simple, repeatable, safe, and hassle free. It would be nice to get it down to an hours worth of work once a month... or scale it up and spend a couple hours once a year.

 

[kinabaloo]

laziness is the mother of invention, so I am a real "MacGyver" type.
For it to be practical, home based WTA extraction has to be simple, repeatable, safe, and hassle free. It would be nice to get it down to an hours worth of work once a month... or scale it up and spend a couple hours once a year.

From where we were at before you came along, this is a major advance - two really: the use of mineral oil and the spiral tube. Puts it within most people's reach.

Btw, have you considered using snufs such as the swedish one with low TSNAs ?

 

[tceight]

I received a PM from a fellow adventurer in HMWTA, and thought it important to post his results ASAP to remind anyone messing around with this to BE CAREFUL and go SLOW. this is uncharted territory, and we do not yet have a means of measuring the strength of the end product.
edited for length and anonymity,

"I dripped a couple of drops into an atomizer and I was like, hmmm, this might not be too bad. Then I think I added 4 drops a couple minutes later and I was sitting here reading something on the forum. My atty leaked from the bottom and I wiped it off, etc., like I do 100 times a day. Suddenly I got this slightly nauseous feeling, but I just ignored it. I kept on reading, not paying much attention to anything, and suddenly I got this feeling like I could pass out or something. The only thing I can compare it to (from a long time ago) is that feeling you get if you've just about had too much alcohol and you want to do this breathing thing like you're giving birth or something, maybe to keep from puking or passing out. I went to the bed quickly so I could lie down. I'm not sure about my pulse because I was scared to check it. I was okay in a couple of minutes."

they also said they did not do the wash step, so may have ended up with a much stronger batch than I did. If so, then this confirms my suspicion of heavy losses of alkaloids in the wash.

 

[tceight]

From where we were at before you came along, this is a major advance - two really: the use of mineral oil and the spiral tube. Puts it within most people's reach.

Btw, have you considered using snufs such as the swedish one with low TSNAs ?

The mineral oil was merely luck because I didn't know better. Looking into it after the fact,by the numbers, alkanes suck. I'm convinced that it was the column 'method' with the mineral oil that allowed it to be successful, and because the oil is not a great solvent, it must give up the alkaloids relatively easily to the PG even with a suboptimal pH.
As for the countercurrent tube... it's a finicky thing to get the bubbles and slope right! arghh!

TSNA's..... mentioned it in the initial extraction post as a consideration in the material selection. I'm pretty certain they do get carried over in this extraction, maybe Dvap can confirm this.
Snus is not readily available to me here, and I like to be 'self sufficient'. I figure a dozen plants should supply all me needs for over a year, and because I am not concerned with the 'quality' of the tobacco, it should be pretty simple to dry and grind up the whole plant. That's my long term plan.

 

[kinabaloo]

TSNA's..... mentioned it in the initial extraction post as a consideration in the material selection. I'm pretty certain they do get carried over in this extraction, maybe Dvap can confirm this.
Snus is not readily available to me here, and I like to be 'self sufficient'. I figure a dozen plants should supply all me needs for over a year, and because I am not concerned with the 'quality' of the tobacco, it should be pretty simple to dry and grind up the whole plant. That's my long term plan.

Might be a way to precipitate them from the WTA extraction with a suitable reaction targeting these amines. Perhaps one of the 'finings'. Perhaps those used in winemaking (partly) to remove amino acids might work ?

 

[bearscreek]

I received a PM from a fellow adventurer in HMWTA, and thought it important to post his results ASAP to remind anyone messing around with this to BE CAREFUL and go SLOW. this is uncharted territory, and we do not yet have a means of measuring the strength of the end product.

That's a bit scary. I had my ground tobacco mixture with the oil in the turkey baster, and after about 12 hours I unexpectedly had to take someone to the emergency room and then to the ICU. Given all this excitement, needless to say, my mixture sat there for 2-3 days. I'm just finishing it up now, so I hope all that extra time didn't create some sort of super-concentrated solution like the one you quoted.

 

[tceight]

so I hope all that extra time didn't create some sort of super-concentrated solution like the one you quoted.

I very much doubt it. For any material in the tobacco, there will be a point of equilibrium reached (depending on several factors). Much like leaving a sponge in a bucket, once it is soaked through, it won't get any wetter.
It would be useful to know just how long that would take, to reduce the extraction time. Heating will speed it up, as will agitation, but if we could know how many hours to let it sit achieve the majority.
A simple 'experiment' someone could try? how about take a droplet every hour, and measure it's opacity? I was thinking a microscope slide, and maybe a light meter from a camera.
Chart the opacity of the droplets, and when they stop getting noticibly 'darker', then we can assume the solvent has done it's work.

mmmm. maybe the same thing could be set up for draining the mineral oil, start sampling the drips as they come through, and see at what point the drips become much clearer, and you know you are done.
damn it...... another project!

 

[BCB]

I received a PM from a fellow adventurer in HMWTA

I THINK I finally figured out what HMWTA is. Home-made WTA? Anyway I'm wondering if the stuff you made gives you the benefits we used to get from cigarettes, that kind of feel-good "ahhh" now that you've had more time to use it? Does it really work?

 

[tescela]

This thread has been awfully quiet lately...

 

[Kurt]

Here is a list of MAO-A and MAO-B inhibitors, for reference.

• Selective MAO-A inhibitors
  • Befloxatone
  • Brofaromine (Consonar)
  • Cimoxatone
  • Clorgyline
  • Curcumin (found in turmeric)
  • Harmala alkaloids (found in tobacco, syrian rue, passion flower, and ayahausca)
    • Harmine
    • Harmaline
    • Tetrahydroharmine
  • Methylene Blue
  • Minaprine (Cantor)
  • Moclobemide (Aurorix, Manerix)
  • Pirlindole (Pirazidol)
  • Toloxatone (Humoryl)
  • Tyrima (CX157)
• Selective MAO-B inhibitors
  • Catechin (found in cat's claw)
  • Desmethoxyyangonin (found in kava kava)
  • Epicatechin (also found in cat's claw)
  • Fo-Ti (active constituent unknown)
  • Hydroxytyrosol (found in olive oil)
  • Lazabemide
  • Pargyline (Eutonyl)
  • Piperine (found in pepper)
  • Rasagiline (Azilect)
  • Selegiline (Deprenyl, Emsam)

Natural MAO-B inhibitors? - Mind and Muscle Forums

A further list, this time primarily botanicals, with some non-overlaps :

◦Selective MAO-A Inhibitors
■Resveratrol (found in skin of red grapes / wine) [13]

◦Nonselective MAO-A/MAO-B Inhibitors
■Curcumin (found in turmeric / curries)
■Harmala alkaloids (found in tobacco, syrian rue, passion flower, ayahausca, and Tribulus terrestris)
■Harmine, Harmaline, Tetrahydroharmine, Harmalol, Harman, Norharman, etc
■Rhodiola Rosea (active consituent(s) unknown)

◦Selective MAO-B inhibitors
■Catechin (found in the tea, cocoa, and cat's claw)
■Desmethoxyyangonin (found in kava)
■Epicatechin (also found in the tea plant, cocoa, and cat's claw)
■Fo-Ti (active constituent(s) unknown)
■Hydroxytyrosol (found in olive oil)
■Piperine (found in pepper)

◦Unknown Selectivity
■Ginkgo Biloba (active constituent(s) unknown)
■Liquorice (active constituent(s) unknown)
■Myristicin (found in nutmeg, parsley, and dill)
■Siberian Ginseng (active constituent(s) unknown)
■St. John's Wort (active constituent(s) unknown)
■Yerba Mate (active constituent(s) unknown)
■Yohimbe (active constituent(s) unknown)

Wapedia - Wiki: Monoamine oxidase inhibitor

"In addition to reversibility, MAOIs differ by their selectivity of the MAO receptor. Some MAOIs inhibit both MAO-A and MAO-B equally, other MAOIs have been developed to target one over the other.

MAO-A inhibition reduces the breakdown of primarily serotonin, epinephrine, and norepinephrine and thus has a higher risk of serotonin syndrome and/or a hypertensive crisis. Tyramine is broken down by MAO-A, therefore inhibiting its action may result in excessive build-up of it, so diet must be monitored for tyramine intake.

MAO-B inhibition reduces the breakdown mainly of dopamine and phenethylamine so there are no dietary restrictions associated with this. Two such drugs, selegiline and rasagiline have been approved by the FDA without dietary restrictions, except in high dosage treatment where they lose their selectivity.[2][4]

Monoamine oxidase inhibitor - Wikipedia, the free encyclopedia

-------

"Monoamine oxidase (MAO) is a mitochondrial outer-membrane flavoenzyme involved in brain and peripheral oxidative catabolism of neurotransmitters and xenobiotic amines, including neurotoxic amines, and a well-known target for antidepressant and neuroprotective drugs. Recently, positron emission tomography imaging has shown that smokers have a much lower activity of peripheral and brain MAO-A (30%) and -B (40%) isozymes compared to non-smokers. This MAO inhibition results from a pharmacological effect of smoke, but little is known about its mechanism. Working with mainstream smoke collected from commercial cigarettes we confirmed that cigarette smoke is a potent inhibitor of human MAO-A and -B isozymes. MAO inhibition was partly reversible, competitive for MAO-A, and a mixed-type inhibition for MAO-B. Two beta-carboline alkaloids, norharman (beta-carboline) and harman (1-methyl-beta-carboline), were identified by GC-MS, quantified, and isolated from the mainstream smoke by solid phase extraction and HPLC. Kinetics analysis revealed that beta-carbolines from cigarette smoke were competitive, reversible, and potent inhibitors of MAO enzymes. Norharman was an inhibitor of MAO-A (K(i)=1.2+/-0.18muM) and MAO-B (K(i)=1.12+/-0.19muM), and harman of MAO-A (K(i)=55.54+/-5.3nM). beta-Carboline alkaloids are psychopharmacologically active compounds that may occur endogenously in human tissues, including the brain. These results suggest that beta-carboline alkaloids from cigarette smoke acting as potent reversible inhibitors of MAO enzymes may contribute to the MAO-reduced activity produced by tobacco smoke in smokers. The presence of MAO inhibitors in smoke like beta-carbolines and others may help us to understand some of the purported neuropharmacological effects associated with smoking."

Herraiz T, Chaparro C.
Spanish Council for Scientific Research, CSIC,
Biochem Biophys Res Commun. 2005 Jan 14;326(2):378-86

Kin, thanks for this list and the refs! I was not aware that resveratrol was an MAOI. I did know it was an anti-inflamatory and antioxidant. Also anticancer. I take it, and would highly recommend it to others, but I've not noticed any obvious MAOI effects. St. John's Wart is thought to be an SSRI, although I don't have refs at the moment.

tceight, really thinking outside the box with this procedure! Nice work! Careful, however, in making sure mineral oil is completely in soluble in PG, VG or water. I don't know the answer to that, and not sure how you would test it outside of a GS-MS or NMR. Even alkanes have a very small solubility in water, and PG or VG will be somewhat similar.

Also, I would highly recommend at least getting a small amount of swedish snus. American, and likely Canadian, tobaccos are fermented, which actually boosts the TSNAs, if I understand correctly (someone can correct me, of course). Swedish is pasteurized, and has very low nitrosamine levels. Swedish snus is also extremely satisfying to me, which tells me I am getting the right balance of tobacco alkaloids, or at least close enough to kill cravings...even to vape, although vaping is just so good that I still will anyway.

I didn't know about CigRX, and its anatabine. The problem I see with concentrating alkaloids, or worse just trying to obtain and vape alkaloids like this, is that the amounts required may be quite small, and much more than that can have very negative effects, such as the myriad side effects that have made pharm MAOIs so unpopular in recent years...bloating, constipation, sedation, and other unpleasant effects.

Additionally, in terms of your extraction, color does not mean alkaloids. There are many coloring compounds in tobacco, such as chlorophyls, in addition to the yellow-brown nic oxides...and I would think nic-like alkaloid oxides, like that of anabatine.

Just my thoughts. Carry on! You are forging new paths in our total learning about all of this!