@IDJoel, That article/video was even too basic for me! From what you have written in places on the forum, you like to get it right, also...What was bugging me goes like this:
1. Luer lock or nipple on end holds some some small amount.
2. Taper section from nipple to barrell holds some small amount.
3. Normally needle holds some small amount. But needs to be subtracted if I don't have a needle on, vs. if I do.
I am assuming that the markings on the syringe start at the bottom of the barrel section, and that the taper on the plunger tip makes up volume of the end taper, so normally you wouldn't have to account for it, as it is taken away from the top of the fluid column. Now I was holding a vacuum in the syringe, and had space in between the plunger and the fluid column, so no way to know when the slowly filling column would stop, exactly, as the viscosity of the liquid keeps it from filling to the plunger tip(in any reasonable time). So, I actually ended up overshooting the .4mark, for instance, which I was aiming for. SO... At this point, I pull syringe from Jug, and have air between fluid column and needle, as well as between plunger and fluid. I was trying to figure out how much I should empty from the syringe to get to exactly .4ml, if that was my aim. Gets a little wierd trying to account for that needle and nipple volume. Normally, yes just push plunger down to expel air, and re-suck fluid back up, but with the small needle, the same thing was going to happen again.
I just used it without the needle, as it was a new bottle, and close to the neck, and that worked this tiime, but got me thinking about what part of the syringe they accounted for when it was marked.
As you can see, I worked it out, even measured the marking length on the syringe to make sure they didn't account for nipple volume.