In my limited experience, I think I figured out how the negative pressure works. Liquid does not expand with lower pressure, and without a bubble you will not have efficient juice feed. The way it works, when you take a puff, the pressure drops in the tank and the air bubble expands pushing the juice into the chamber. The bigger the bubble, the more it expands, and more juice makes its way into the chamber. That's half the story. The air expansion has to be big enough so when you stop the puff, and the air bubble contracts it contracts enough to suck the juice out of the juice channels and also sucks some air from the chamber into the tank to equalize the pressure again or your next puff you will get a dry hit.
I guess one way of looking at it. If you have a 10ml bubble and you create enough negative pressure to expand it by 10% you will inject 1ml of juice into the chamber (10% of 10ml = 1ml) on the other hand, if you have a 100ml bubble and expand it by 10%, you will inject 10ml of juice into the chamber (10% of 100ml = 10ml)
Just by looking at the above example it becomes clear that a bigger bubble = more juice in the chamber.
When I fill the lemo, I fill it upside down until it reaches the chamber. That leaves a perfect bubble for me to start with. As the juice drains and the bubble increases I get better feed and can easily increase the wattage.
Just my observations. Could be wrong and way off base.
Edit: by covering the air holes when taking the puff or adjusting the air flow, you create a bigger pressure drop and the bubble expands more, air volume (bubble size) is inversely proportional to pressure. So you compensate for the smaller bubble with larger pressure drop. In the above example the bubble expansion could possibly be 15% with the lower pressure. 10% IMHO is very liberal and unrealistic, but it is a nice round number for the example.