there is too many variables to do anything as far as needing the high cost test.. and it would have to be ppl that avoid areas with a lot of smoke.. the tests do not pick up low amounts of nic I have smoked a analog 1 hour before taking one of these and it did not pick it up.. 
would not have to be a set mg use or set amount would be average usage since this is just to see nicotine absorption taking place.
	
		
			
		
		
	
			
			would not have to be a set mg use or set amount would be average usage since this is just to see nicotine absorption taking place.