Sterilization in homemade E-Juice and equipment
- Thread starter Isaiah Rodriguez
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OK, smoked - I like you, and enjoy your posts. Most are pretty intelligent (although your posts about disassembling and cleanliness can remind me of Bill Hartman's SNL ".... Retentive Chef" skit
). But this post was a little more personal, seemed an attempt to ridicule me (emojis notwithstanding), and the analogy isn't your best work.I don't go through the extra work because there's simply no benefit (that I'm aware of) to it. If I don't wipe, I'm pretty sure I'll suffer immediate and somewhat lasting repercussions (as will those in my immediate vicinity). Just as I don't routinely completely disassemble/sterilize/reassemble my coffeemaker/car/computer/you name it, I choose not to spend time going beyond what is necessary to clean my vaping equipment, and have suffered no consequences. What byproducts are you concerned about, exactly, and what do you believe they are going to do to you? I'd bet dollars to donuts that I could present you with two atties, one hot-rinsed, and one disassembled/washed/sterilized/reconstructed to your specifications, and you couldn't vape them and tell me which is which....even after months of use. Is there some vaping byproduct the rest of us haven't heard of that you are trying to avoid?
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Im with ya on that. Clean an ashtray. Why? It will be full of ashes anyway.Heck, you shoulda seen what my ashtrays used to look like!
I'm someone who rarely cleans an attie past a quick rinse in water from the sink when I dry burn, so I'm obviously not too worried about microorganisms in my vape
However, I don't think the vaping process does much to kill off microorganisms. The problem is the time spent at high enough temperature to kill them is just not long enough, or the juice doesn't get hot enough for even a short time. IMO if our juices grew enough microorganisms fast enough to become a problem, it would be a problem for us unless we did something to control it. If this was the case we would all know about it.
I've vaped juice that was over two years old and stored at room temperature, used small amounts of lemon extract that was two years old and stored the same way and have never has an issue that I am aware of.
In my experience juice with our typical ingredients simply does not 'go bad' at room temperature on the shelf or in an attie.
I agree with everything they said in the video. As I donned my tyvec suit, gloves and respirator, I stepped through the airlock into my iso 7 lab to make my morning coffee. I was thinking how most of the population gamble with their lives making food and drinks out in the open. People have turned into everyday daredevils. 
In the past I was a devil may care kind of guy. Prior to this thread, I never worried about my bacterial laden liquid nor my contaminated atomizers. My eyes have been opened and you, my friend Asbestos, have become an inspiration to us all. No half measures for you!
So, um, no 5 second rule at your house?
Drop on floor rule at my house is if I get to it before the critters it's gold. Joe is still with us, he woke me up last night eating.
I read this article awhile back. https://amp.usatoday.com/amp/355947...ARAB&usg=AOvVaw1hEBzjI2f3t1R08OQ97hne&cf=1
That being said, I dont really much care because bacteria is everywhere and I dont use cotton I use cellucotton (rayon). I dont trust even organic cotton because pesticides can remain in the soil for 50 years or more. I wrote a long protest about it on here a couple years back.
The study (not the article) is interesting, and a little odd. First, even though it's a recent study, they chose the most popular products from 2013. They're testing for beta D-glucan, which itself isn't a harmful substance, but used as a marker to detect potential fungal infection. But Beta Glucan is actually used to promote the immune system to treat many things, such as high cholesterol, diabetes, allergies, hepatitis, Lyme disease, eczema, and even cancer. So, not being a medical researcher, I'm not quite sure what their presence in eliquid means.
And endotoxins - this publication states that it is an important component in the inflammation process, it also states that repeated exposure to low levels of endotoxin actually increases immunity.Endotoxin - an overview | ScienceDirect Topics
Endotoxin Tolerance
Endotoxin tolerance, mentioned earlier in relation to the airways, is another mechanism that is important in the gut (Fig. 2). Animals can survive a potentially lethal dose of endotoxin if they have previously received one or more sublethal doses, and in vitro macrophages that have been exposed to endotoxin respond differently when challenged again, with less release of TNF and reduced NF-κB translocation (Biswas & Lopez-Collazo, 2009). Repeated low-dose endotoxin administration in vivo leads eventually to increased Treg activity (Caramalho et al., 2011; Wang et al., 2015).
So I don't know...as always, I believe it'd be better to breath nothing but air. Seems the presence of these substances, like much medical news, gives contradictory info.
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