To be honest I have developed my methods and technique quite a bit sense my original post... I left this place for quite a while due to some disagreements I have had with others among the community. It seemed when I was working on this around here people were more concerned over childish definitions and personal reputations instead of actually achieving something...
It seems though the environment has changed a little bit, maybe as the federal government taking away our nicotine becomes a more and more serious threat there may develop more interest in DIY extracts... I have always been interested because pure nicotine did not completely solve my tobacco cravings, no matter how high I put the nicotine. Currently I am convinced that what I consume does solve my tobacco cravings, and it is a synergistic effect of tobacco alkaloids being consumed that satisfies these cravings for me.
Anyways, with regards to my current process... I am not recommending this or making any claims, I try my best not to give advice anymore, this is simply what I find works best for me. Be careful if you are playing with nicotine, the stuff is quite dangerous.
1: Extract tobacco with room temperature Isopropyl Alcohol, 91%. Usually I do 3 pulls, about 2 hours per pull.
2: Evaporate it down to ~50%, I then freeze if for 12 hours and then filter it while it is still ice cold. The precipitate is plant gunk.
3: Evaporate it down to syrup.
4: Take up the syrup with minimal room-temp grain ethanol.
Not Isopropyl Anything of marginal solubility is no-good plant gunk.
5: Freeze it for 12 hours, filter/decant while cold, then evaporate to syrup. Again the solids are plant gunk.
6: Repeat step 4-5 at least 1 more time or until you can get nothing to crash out... But be aware each time has some minor losses of alkaloids.
If you take repeat step 6 enough times, and dissolve the product in a minimal enough amount of solvent you may get the alkaloids to crystallize. This would require a very concentrated solution, and very low temperatures, and most likely a solvent other than ethanol. However, it may be possible with ethanol as it becomes pure, if you are on the second and beyond time of steps 4-5, and you get a bunch of crystalline precipitate, don't throw it away...
I start with isopropyl because it is cheaper makes it far less cost prohibitive. Then taking it up in ethanol and evaporating that will result in isopropyl free final product.
Also in regards to making the process less cost-prohibitive, if you own or setup a basic vacuum-distillation setup it is very realistic to save the majority of your solvents. It is also possible to acquire much higher purity solvents by first distilling the over-the-counter sources.
I am personally very comfortable with consuming this final product, it barely gunks up my coils (actually I think less so than commercial ejuice...) but I don't add any flavoring or other garbage to it... Just the ethanol extract added to pharma grade VG/PG... Based on personal experience I am quite confident the resulting product has a strong tobacco effect, and satisfies my personal craving for a cigarette quite unlike regular ejuice does.
Call it a NET if you must, sure it may or not be a 'WTA' based on various definition of terms, but it most certainly contains all the goodies the tobacco had to offer, and it is as pure as I can achieve without getting into far more complicated methods. (Distillation, Acid/Base Extraction, etc.) I find that the Acid/Base approach is far to sketchy to realistically achieve with over the counter methods to any level of purity I'm comfortable with consuming.
Regarding the testing of this extraction, cessnapix you are correct that an HPLC would most likely be needed in order to verify the results. Something I have yet to procure... You could get a pretty good estimate of the alkaloid content by doing a titration only you will have to be working in the opposite direction, because ~90% of your alkaloids are currently already in the form of neutral salts when in tobacco. Unless you treated your tobacco or extraction with a base at some point? Anyways it starts to get messy and based on all kinds of unverifiable assumptions at that point, so IMO it isn't worth doing.
Putting all the alkaloids in a basic solution would indeed increase the bio availability and the intake of the alkaloids... At least in theory. However in the salt form it would be far more stable and less likely to evaporate! Freebased nicotine is indeed quite volatile, and will easily evaporate away with a solvent if not evaporated under vacuum... I found this out the hard way with a few distillation experiments, and verified it through research... So pretty much you need a quality lab equipment in order to achieve pure freebased nicotine.